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Tunable Femtosecond Lasers for Bioimaging: Balancing Energy and Power

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Tunable Femtosecond Lasers for Bioimaging: Balancing Energy and Power

Presented by: Dariusz Swierad, Fluence.technology

Femtosecond lasers for two-photon excitation microscopy now feature tunable repetition rates, optimizing the energy-to-average-power ratio for superior imaging performance. These tunable lasers allow precise adjustment of pulse energies, minimizing photobleaching and maximizing imaging depth. By enabling operation at optimal conditions, these lasers improve image quality and reduce damage to biological samples. The capability of fine-tune repetition rates ensures high photon densities without excessive heat buildup, making them ideal for advanced in-vivo imaging and extended observation periods.


About the presenter
Dariusz SwieradDariusz Swierad, Ph.D., is an experienced executive in photonics and adjacent industries, working in management positions from engineering to sales and marketing. Since 2018, he has been director of sales and marketing at Fluence.technology, a company developing and manufacturing next-generation industrial grade, reliable, long-lifetime, serviceless, ultrafast femtosecond laser technology.

From 2017 to 2018, Swierad led the development of a miniaturized atomic clock at Teledyne e2v, and prior to that, he was a field sales engineer at TOPTICA Photonics UK, developing the laser market for quantum and terahertz applications. He is a physics graduate from the Nicolaus Copernicus University in Torun in Poland, where he also worked at the National Laboratory for Atomic, Molecular and Optical Physics (KL FAMO). Swierad was awarded a doctorate from the University of Birmingham in the U.K., where he developed stable and ultrastable laser systems for optical atomic clocks, including the Space Optical Clock for the International Space Station.




About the sponsor(s)
  • Fluence sp. z o.o. - Manufactures femtosecond lasers that are environmentally stable and immune to shock. Provides service-free femtosecond lasers for micromachining. Offers stability and environmental resilience of lasers with a robust all-fiber technology.


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