Analysis of Common Dyes Using Steady-State and Time-Resolved Spectroscopy

Modern hematology and histology rely heavily on various dyes and stains to understand what is happening in samples of blood and biological tissue. The systems and components used to analyze these colorimetric and fluorescent compounds are just as vast as the families of dyes, and within a single system there are also multiple methods of processing the same data feed. Guenther presents a range of common hematological and histological compounds — such as 5(6)-carboxy-2,7-dichlorofluorescein (CDCF), sudan III, methyl red, and 4-(2-pyridylazo)resorcinol (PAR) — analyzed with several genres of spectroscopic systems. He makes comparisons between steady-state broadband intensity and time-resolved fluorimetry methods, and within these techniques, he further explores the data processing options. Guenther also discusses the fiber optic components available for interrogating biological samples, and he completes the full systems used in this type of analysis.

About the presenter
Derek Guenther has a bachelor’s degree in chemical engineering, with a minor in inorganic chemistry, from the University of South Florida (USF). His work at the USF Institute for Environmental Studies focused on novel silica-based algaecides targeting invasive species. Guenther’s strengths and passions lie in new chemical sensor development and spectral numerical processing. He has spent over 12 years at Ocean Insight developing and supporting the optical oxygen, pH, and SERS-based sensor products, and today he works with the Lab Services Team helping customers achieve their goals.