Expanding Capabilities with Optimized NIR Confocal Imaging

Fluorescence microscopy historically has been dominated by blue, green, and red imaging. Although this limitation is acceptable for some applications, multiplexing and deep tissue imaging suffer with blue to red as the only imaging window. This limitation of imaging into the blue-to-red window was due to many factors, but primarily these three: 1) fluorophores were not widely available for far-red and near-infrared imaging; 2) lasers and detectors were not optimized for far-red imaging; 3) optical coatings were poor at transmitting into the far-red and near-infrared wavelengths. Lopez discusses recent scientific advancements in all three of these areas and highlights how new capabilities can offer unique benefits for multiplexing and deep tissue imaging.

About the presenter
James Lopez, Ph.D., received his doctorate in biomedical sciences from the University of Chicago in 2010. With nearly a decade of experience in the areas of calcium imaging, Förster resonance energy transfer (FRET), live-cell imaging, and intravital imaging, he joined Olympus as a confocal and multiphoton sales representative. He later transitioned to the Olympus Life Science Applications Group supporting confocal and multiphoton systems. Now he manages the Life Science Applications Group in the U.S., Canada, and Latin America markets.