Scaling Functional Brain Imaging with Advanced High Power Multiphoton Microscopy

In recent years, advancements in neuroscience have been nothing short of revolutionary. Advanced two- and three-photon imaging methods, once the domain of pioneering laboratories, are becoming increasingly sophisticated and mainstream in research settings. In addition, core facilities have started adapting two-photon and three-photon microscopy to provide imaging services to users. Robustness and reliability of the laser source are the main factors for success in this realm.

Holographic techniques, though demanding in terms of peak power, open new routes in imaging, while fiber-coupled miniscopes provide versatility in real-time, in-vivo visualization. Adaptive optics have taken center stage, delivering sub-cell resolution, and elucidating the nuances of neuron-axon interactions.

This presentation will spotlight how high-power, high-repetition rate lasers are integral to the next generation of systems, amplifying the scientific community's understanding of brain activity, from individual neuronal dynamics to comprehensive ensemble interplay.

About the presenter
Kolja Kolata, Ph.D., earned a doctorate in physics from University of Marburg, Germany, in 2014 with a focus on ultrafast pump–probe spectroscopy on organic semiconductors provided the ideal foundation for launching a technical career in industry.

After seven years as a sales engineer at SmaAct GmbH helping build deep expertise in technical sales to both industrial and academic customers, Kolata joined Class 5 Photonics in 2022.

Starting as a sales engineer, Kolata became a sales manager at the beginning of 2025, driving customer-focused solutions and business growth in advanced laser technologies.