How to Use a Fluorometer to Characterize Biomolecules

Fluorescence spectroscopy is a powerful technique in biophysics, providing highly sensitive and non-invasive access to the molecular behaviour of biological systems. By probing parameters such as emission spectra, fluorescence lifetimes, and photoluminescence quantum yields, it enables detailed characterisation of molecular environments, interactions, and dynamic processes.

This talk offers a practical overview of the essential considerations when applying fluorescence methods to biologic samples. It will highlight how instrument configuration, measurement strategies, and experimental design can be tailored to support the development, evaluation, and application of fluorescent probes and building blocks in biological research.

About the presenter
Michele Duarte Tonet, Ph.D., received her doctorate from the University of St Andrews under the supervision of Professor Graham Turnbull and Professor Eli Zysman-Colman. During her studies she focused on photoluminescent sensors using different classes of emitters, gaining expertise in fluorescence spectroscopy.

In 2026, Tonet joined Edinburgh Instruments as an applications scientist, specializing in fluorescence, where she creates scientific content, supports researchers with technical support and training, and assists with experimental design and data analysis.