Opening New Frontiers for Multiphoton Microscopy with Advanced Laser Technology

Over the past three decades, multiphoton microscopy has become a workhorse for monitoring biological processes at subcellular resolution deep within live tissue and animals. Today, the fields most commonly relying on multiphoton microscopy are neuroscience and cancer research.

Despite numerous advantages, limitations in microscope and laser technology have hindered the application of multiphoton microscopy to many research questions due to trade-offs in imaging speed, field of view, and imaging depth.

The past decade has seen the emergence of numerous novel multiphoton microscopy techniques that are pushing beyond those initial boundaries. These include three-photon microscopy for deeper imaging, scanless two-photon imaging for high frame rates, and the combination of imaging and photostimulation for all-optical interrogation of neuronal circuits. Most of these advancements have gone hand in hand with advancements in laser technology, allowing for wider adoption among application-oriented researchers.

In this presentation, we will explore examples of how recent advancements in laser technology have helped to overcome previous limits in multiphoton microscopy.

About the presenter
Christian Wilms is a neuroscientist by training, and has spent over one and a half decades in the lab, applying two-photon imaging and uncaging to a range of questions. After several years spent as part of the R&D team at a company producing multiphoton microscopes, he joined Light Conversion in 2025. Here, he is the market development manager for biomicroscopy. He specializes in nonlinear microscopy, with a particular focus on two- and three-photon imaging.