Block the Laser, Keep the Signal: Filter Specifications that Make or Break Multiphoton Images

Multiphoton microscopy requires optical filters to do something that no other fluorescence technique requires: reject high-power, pulsed near-infrared light while passing visible photons. This presentation walks through the detection path of a multiphoton system and identifies the specification decisions that most often limit image quality, including blocking depth and blocking range across a tunable source, angle-of-incidence effects in scanned detection, dichroic flatness and group delay dispersion in the excitation path, and channel placement for second- and third-harmonic signals.

Drawing on filter selection work with multiphoton labs and instrument builders, Kinoshita reviews the specifications worth confirming before ordering a filter, and the cases where a stock part will not do the job.

About the presenter
Roy Kinoshita, Ph.D., is an account manager for microscopy at Chroma Technology Corp., a leading manufacturer of precision optical filters. He plays a crucial role in supporting customers across the biotechnology and photonics industries.

Kinoshita is known for his ability to understand complex technical requirements and deliver tailored solutions that meet client needs. His expertise contributes to Chroma's mission of providing high-quality optical components for life sciences applications such as widefield fluorescence, confocal, multiphoton, and super resolution. His commitment to customer satisfaction and his collaborative approach have made him a valuable asset to the Chroma team.