Setting the Standard: Performance Requirements for Fluorescence Imaging

Fluorescence imaging systems span a wide gamut of cost and complexity, with components that can range from drop-together plastic assemblies to precision-aligned immersion microscope objectives. Where a system lands in this space is primarily driven by the required performance. That’s why it’s critical to correctly define the necessary requirements for an application.

In this webinar, Masella discusses several specifications that determine the complexity of fluorescence imaging systems, including field-of-view, numerical aperture, sensitivity, and resolution. He also explores how these choices can drive design direction and system complexity, and how to determine whether a requirement is over-specified for a given application.

About the presenter
Ben Masella, Ph.D., is a principal optical engineer at Optikos Corporation, where he leads optical design, tolerancing, and system development for advanced imaging and metrology solutions. He previously served as a senior optical engineer at Optikos and as an optical engineer at Janos Technology, specializing in mid-wave and long-wave infrared optics, including aspheric and diffractive designs. Masella’s expertise spans sequential and non-sequential modeling using Code V, Zemax, and FRED, with supporting programming experience. He earned a doctorate in optics from the University of Rochester, where his research focused on adaptive optics retinal imaging and phototoxicity, and holds a bachelor’s in physics from Tufts University.