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Photonics Spectra Conference 2021

Rapid and Effective Large-Area Raman Imaging: Raman Microscopy Evolves

Presented by: Andrew Whitley, HORIBA Scientific

The last 25 years have seen Raman microscopy evolve from a relatively slow spectroscopic imaging technique, which could take days to image a sample, to an accepted rapid and powerful technique. It is now standard for Raman microscopes to generate full spectroscopic large-area 2D and 3D maps/images in minutes. Images of whole pharmaceutical tablets and full semiconductor wafers and devices are now commonplace. The inherently low-scattering cross section of Raman spectroscopy (RS), as well as its diffraction-limited lateral resolution, have been overcome by new Raman components and microscopy techniques. In some cases, video-rate Raman imaging is becoming a reality. At the same time, beautiful Raman images on a nanometric scale are possible by utilizing the combined technique of Raman and atomic force microscopy (AFM) through the application of tip-enhanced RS (TERS). These capabilities have become possible not only through advancements in Raman components and spectrometer design but also through the implementation of smart selective sampling and multivariate analysis software. Recently, the use of novel Raman reporter molecules with SERS or isotopic tags has allowed increases in both specificity and sensitivity.

In this presentation, Andrew Whitley reviews the current methods and tools employed in Raman imaging. Enabling technologies include Raman microscopy, spatially offset RS (SORS), stimulated RS (SRS), particle-correlated RS (PCRS), and automated region-of-interest smart selective Raman mapping. Applications illustrated include large-area mapping of pharma samples, in vivo imaging of cancer through the intact skull of small animals, DNA imaging and sequencing, and nanometric imaging for the characterization of 2D materials.


About the presenter
Andrew WhitleyAndrew Whitley, Ph.D., received his doctorate in chemistry from the University of Durham in England. Andrew's career has focused on the development of spectroscopic instrumentation, specializing in molecular spectroscopy in particularly Raman and fluorescence spectroscopy. He is currently the VP for Global Business Development at HORIBA in NJ, where he has worked since 2000. Andrew is responsible for a global team focusing on developing dedicated applications specific products for high volume business. Andrew has authored many papers, articles and book chapters describing the use of spectroscopy. He is a regular presenter and session organizer at SciX, Pittcon, IFPAC and other global conferences. At SciX, Andrew has organized many sessions on varied spectroscopic topics. In 2018 Andrew became the Marketing Chair for the Society for Applied Spectroscopy, responsible for membership, publicity, web and social media. The same year Andrew was awarded the Mann Award by FACSS. In 2021 Andrew will be President Elect of SAS, and will become SAS President in 2022. Recently Andrew has been heavily involved in the development of analysis methods for microplastics. In 2019 he co-organized a major workshop on MP's at SCCWRP in CA, organized two special SAS sessions on MPs at SciX, and served as associate editor for the September, 2020, special issue of Applied Spectroscopy on microplastics.




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